Test principle of spot human binding globin ELISA detection kit

Shanghai Enzyme Biotechnology Co., Ltd. specializes in supplying ELISA kits, kits, detection kits, human ELISA kits, rat ELISA kits, mouse ELISA kits, ELISA kits, ELISA KIT, rabbit ELISA kits, guinea pigs ELISA kit, cattle ELISA kit, sheep ELISA kit, dog ELISA kit, pig ELISA kit, hamster ELISA kit, kit, detection reagents, antibodies, cell lines, culture medium, tumor necrosis factor, interleukin 2, Interferon, standards, controls, biological reagents, markers, inhibitors, antigens, agar, cell growth factors, etc. Human binding globin ELISA test kit II. Precautions This reagent is an in vitro test reagent. It should be used within the validity period. The reagent should be regarded as an infectious agent. Before use, the reagents in the box should be taken out and left at room temperature for at least 30 minutes. Store at 2-8 ° C, do not freeze, please see the label in the box for the expiration date. After the concentrated washing solution crystallizes, incubate at 37 ° C for 15 minutes. The human-bound globin ELISA detection kit III. Operation steps Add 100ul of the diluted sample and the standard respectively to each well. The plate was incubated at 37 ° C for 30 minutes. Shake off the liquid in the plate, wash with the application washing liquid, stay for 30 seconds each time, pat dry on the paper. Repeat the operation 5 times. Add 100ul of enzyme-labeled coupling solution to each well. The plate was incubated at 37 ° C for 30 minutes. Shake off the liquid in the plate, wash with the application washing liquid, stay for 30 seconds each time, pat dry on the paper. Repeat the operation 5 times. Add 50 ul each of Substrate A and Substrate B to each well, and place in a 37 ° C incubator for 15 minutes. 8. Add 50ul of stop solution to each well and read the OD value at 450nm. Galectin galectin antibody 0.2ml GAP-43 (Growth associated protein-43) nerve growth associated protein-43 (antibody) 0.1ml GAP-43 (Growth associated protein-43) nerve growth associated protein-43 (antibody) 0.2mlGFP (Green Fluorecent protein) Green fluorescent protein 0.1ml GFP (Green Fluorecent protein) Green fluorescent protein 0.2ml GHRF / GHRH growth hormone releasing hormone antibody 0.1ml GHRF / GHRH growth hormone releasing hormone antibody 0.2ml phospho GAP-43 (pSer41) (phospho Growth associated protein 43 ) Anti-phosphorylated nerve growth related protein 43 antibody 0.2ml GAPDH glyceraldehyde 3-phosphate dehydrogenase (antibody for rabbit-derived immunohistochemistry) 0.1ml GAPDH glyceraldehyde 3-phosphate dehydrogenase (antibody for rabbit-derived immunohistochemistry) 0.2mlGαi -3 (G alpha i-3) Inhibitory G protein α3 antibody 0.2ml CXCR3 / CD183 cell surface chemokine receptor 3 antibody / G protein coupled receptor 0.2ml IV. Results judging instrument value: enzyme label at wavelength 450nm Read the OD value of each well on the instrument. Detection value range: 0-800mg / L Sensitivity: 1.0mg / L

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